1976-1980 Nanyang Technological University, Bachelor
1980-1983 West Virginia University, Master
1983-1987 Cornell University, Ph.D.
Research Areas
Plant pathology, virology, molecular biology, structural biology, biotechnology, nanotechnology.
Research Interests
Research Accomplishments
1. Determination of full-length sequences and generation of biologically-active cDNA clones of two most prevalent orchid viruses – CymMV and ORSV.
2. Discovery of two novel open reading frames from HCRSV and a polyA tract in HLSV.
3. Molecular characterization of AYVV, CMV and ZYMV.
4. Successful application of non-radioactive cRNA probes, polymerase chain reaction, capillary electrophoresis, molecular beacons and quartz crystal microbalance for plant virus detection.
[1] Liu HQ, Zhou YJ, Xu QF and Wong SM. (2020). Selection of DNA
aptamers for subcellular localization of RBSDV P10 protein in the midgut of
small brown planthoppers by emulsion PCR‐based SELEX. Viruses 12, 1239.
doi:10.3390/v12111239.
[2] Wu C, Kannan S, Verma CS, Swaminathan K and Wong SM. (2020). Molecular modeling
and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip
crinkle virus coat protein. Virology 551: 64-74.
[3] Yang X, Das PP, Oppenheimer P, Zhou GH and Wong SM. (2020). iTRAQ-based
protein analysis provides insight into heterologous superinfection exclusion
with TMV-43A against CMV in tobacco (Nicotiana benthamiana) plants. J
Proteome 229: 103948. https://doi.org/10.1016/j.jprot.2020.103948.
[4] Guo S and Wong SM. (2020). Small RNA derived from Tobacco mosaic virus targets a
host C2-domain abscisic acid-related (CAR) 7-like protein gene. Phytopathol.
Res. 2: 15. https://doi.org/10.1186/s42483-020-00058-7.
[5] Yang ZT, Wong
SM and Yue GH. (2020). Characterization of GAB3 and its association with NNV
resistance in the Asian seabass. Fish & Shellfish Immunol.104:
18-24.
[6] Ren R, Gao J, Lu CQ, Wei YL, Jin JP, Wong SM, Zhu GF and Yang FX.
(2020). Highly
efficient protoplast Isolation and transient expression system for functional
characterization of flowering related genes in Cymbidium orchids. Int.
J. Mol. Sci. 21, 2264; doi:10.3390/ijms21072264.
[7] Yang ZT, Wang L, Wong SM and Yue GH. (2020). The HIF1αn gene and its association
with hypoxia tolerance in the Asian seabass. Gene 731: 144341. https://doi.org/10.1016/j.gene.2020.144341
[8] Guo S and Wong SM. (2020). A conserved carboxylesterase inhibits Tobacco mosaic
virus (TMV) accumulation in Nicotiana benthamiana plants. Viruses 12, 195; doi:10.3390/v12020195
[9] Macharia M, Das PP, Naqvi NI and Wong SM. (2020). iTRAQ-based
quantitative proteomics reveals a ferroptosis-like programmed cell death in
plants infected by a highly virulent tobacco mosaic virus mutant 24A+UPD. Phytopathol.
Res. 2:1. https://doi.org/10.1186/s42483-019-0043-5
[10] Wu W, Liu HQ, Dong Y, Zhang Y, Wong SM, Wang CC, Zhou YJ and Xu
QF. (2019). Determination
of suitable RT-qPCR reference genes for studies of gene functions in Laodelphax
striatellus (Fallén). Genes. 10.887. doi 10.3390/genes
10110887
[11] Guo S and Wong SM. (2019). Poly(A) introduced upstream of the upstream pseudoknot
domain of Tobacco mosaic virus led to sequence deletion after serial passaging
in host plants. Phytopathol. Res. 1:24.
[12] Macharia MW, Tan WYZ, Das PP, Naqvi NI and Wong SM. (2019). Proximity-dependent
biotinylation screening identifies NbHYPK as a novel interacting partner of
ATG8 in plants. BMC Plant Biology 19:326.
[13] Das PP, Macharia MW, Lin QS and Wong SM. (2019). In planta proximity-dependent biotin identification (BioID) identifies a TMV replication co-chaperone NbSGT1 in the vicinity of 126 kDa replicase. J Proteome 204: 103402
Website
https://www.dbs.nus.edu.sg/staffs/wong-sek-man/?cid=59 ?
